Journal: mBio

Article Title: HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like Tail

doi: 10.1128/mBio.01272-17

Figure Lengend Snippet: Nucleoid staining of HupB-EGFP and HupB-mCherry strains. (A and B) DAPI staining of WT and HupB-EGFP cells from log phase and stationary phase, respectively. (C and D, top panels) SYTO 45 staining of HupB-mCherry cells from log- and stationary-phase cultures, respectively. (Bottom panels) Fluorescence profiles along the long cell axis, as measured from the distant cell pole of SYTO 45-stained HupB-mCherry cells from log-phase ( n = 50) and stationary-phase ( n = 50) cultures, respectively. Scale bar, 2 μm.

Article Snippet: We next examined the numbers of HupB-EGFP monomers per cell by performing Western blotting of total M. smegmatis cell proteins with antibodies raised against EGFP, using purified recombinant EGFP (rEGFP; Cell Biolabs) to generate a standard curve.

Techniques: Staining, Fluorescence

Journal: mBio

Article Title: HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like Tail

doi: 10.1128/mBio.01272-17

Figure Lengend Snippet: Real-time visualization of HupB-EGFP-DNA macrocomplexes. (A) Time-lapse analysis of sister chromosome separation during the cell cycle. t 0 indicates sister chromosome separation, and a red triangle marks each separation event. Scale bar, 2 μm. (B) Graph presenting the real-time localizations of HupB-EGFP-DNA macrocomplexes in a representative cell. The fluorescence intensity along the long cell axis was measured from the old cell pole. Here, t 0 does not correspond to sister chromosome separation but rather to the middle of the cell cycle. (C) Chart showing real-time mean chromosome condensation levels with corresponding 95% confidence intervals. Chromosome condensation is shown as a percentage of cell length and was measured between two sister chromosome separation events ( n = 30).

Article Snippet: We next examined the numbers of HupB-EGFP monomers per cell by performing Western blotting of total M. smegmatis cell proteins with antibodies raised against EGFP, using purified recombinant EGFP (rEGFP; Cell Biolabs) to generate a standard curve.

Techniques: Fluorescence

Journal: mBio

Article Title: HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like Tail

doi: 10.1128/mBio.01272-17

Figure Lengend Snippet: Subcellular localizations of HupB ΔCTD -EGFP and HupB-EGFP. (A) Micrographs showing representative cells of the analyzed strains. (B) EGFP fluorescence profiles of representative HupB-EGFP and HupB ΔCTD -EGFP cells along the long cell axis, as measured from the distant cell pole. Scale bar, 2 μm.

Article Snippet: We next examined the numbers of HupB-EGFP monomers per cell by performing Western blotting of total M. smegmatis cell proteins with antibodies raised against EGFP, using purified recombinant EGFP (rEGFP; Cell Biolabs) to generate a standard curve.

Techniques: Fluorescence